Submission Guidelines

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Submission guidelines: All specimens submitted to lab must be accompanied by a


Recommended protocol for submitting tissues:

Formalin fixed tissue:

Fixation should be carried out as soon as possible after removal of tissue to prevent autolysis. The most common fixative in use is 10% neutral buffered formalin.  For adequate fixation, the preferred thickness of tissue is 3 to 5 mm. Ideally the fixative should be ten times the volume of the tissue. Specimens need to be fixed for a minimum of 24 hours (longer for larger specimens) for adequate fixation before additional processing.

Once tissue is adequately fixed, bring them in to the lab for processing and further steps.  Make sure the specimen labels are easy to read and firmly adherent to the specimen containers.   

Please let us know any special instructions to be followed for your sample in terms of  trimming, processing and sectioning.

New users are encouraged to contact the Core Lab to discuss their objectives and sample requirements before harvesting samples, as well as for advice on best tissue harvesting and fixation methods.


For each submission we request that you submit positive and negative controls (tissues, slides or blocks) along with the primary antibody.


Soon after the tissue is dissected, place it in the labeled mold with OCT medium and flash-freeze in a bath of isopentanethat is  immersed in liquid nitrogen or dry ice. Orientation of the tissue needs to be considered before freezing. Frozen tissue should be stored in -80C until it is sectioned.

Any deviation from the above protocol is to be mentioned in the service request form.  We can help you to determine the best method for demonstrating the particular antigen of interest in a specific tissue.

Transmission Electron Microscopy (TEM):






Fixation is a most critical step in electron microscopy. Proper fixation ensures preservation of the ultrastructure of the sample. If necessary, the Core Lab can provide a standard fixative (2% gluteraldehyde, 1% paraformaldehyde in 0.1M cacodylate buffer at pH 7.2)  and clear instructions for each step. The tissue should be cut into  pieces no larger than 3 mm on any side. Identifying labels need to be clearly written on the container and the type of fixative and length of fixation should be included.







Please contact the Pathology Core Laboratory to coordinate collection and/ or storage of frozen tissues well in advance of the need to insure that any required authorizations or consents are in place and the laboratory understands the special handling that may be required. Please inquire about pricing.