Meet The PI

Alberto BosqueAlberto Bosque, Ph.D., M.B.A., joined the George Washington University in November 2016 as Assistant Professor in the Department of Microbiology, Immunology and Tropical Medicine. He is a biochemist with considerable experience in cell biology and signal transduction. 

Dr. Bosque received his Bachelor’s degree in Biochemistry with high honors from the University of Zaragoza, Spain in 2001. During his graduate studies at the University of Zaragoza in the laboratory of Dr. Alberto Anel, he studied the mechanisms of downregulation of human T cell immune responses and their implication to Autoimmune Lymphoproliferative Syndromes (ALPS). 

After obtaining a Ph.D. in Biochemistry and Molecular and Cellular Biology with honors “cum laude”, Dr. Bosque moved to the U.S. and joined the laboratory of Dr. Vicente Planelles at the University of Utah, first with a postdoctoral fellowship from the Spanish Government followed by a Mathilde Krim Postdoctoral Fellowship from The Foundation for AIDS Research (amfAR). During his postdoctoral studies, he developed one of the first primary cell models of HIV-1 latency. In 2011, Dr. Bosque became a Research Assistant Professor at the University of Utah and in 2015 an Assistant Professor. During his time as Faculty at the University of Utah, he also completed with honors a Master Degree in Business Administration (M.B.A.).

Dr. Bosque joins MITM and the Research Center for the Eradication of HIV as an Assistant Professor, where he will continue using this model of latency to discover and characterize several latency reversing agents as well as to study the mechanisms involved in the establishment of HIV latency.

Honors and Awards

2009 - Mathilde Krim Postdoctoral Research Fellowship, The Foundation for AIDS Research (amfAR), USA.

2007 - Postdoctoral Research Fellowship, Ministry of Science and Technology, Spain.

2006 - Award for the most outstanding graduate student in Health Sciences, University of Zaragoza, Spain.

2002 - Graduate Research Fellowship, Ministry of Science and Technology, Spain.

2001 - Award for the most outstanding graduate student in Biochemistry, University of Zaragoza, Spain.

2000 - Undergraduate Research Fellowship, University of Zaragoza, Spain.

Previous Contributions to Science

A. Characterizing the role of pro- and anti-apoptotic proteins in the downmodulation of T cell immune responses.

1. Bosque A, Pardo J, Martinez-Lorenzo MJ, Iturralde M, Marzo I, Pineiro A, Alava MA, Naval J, Anel A (2005). Down-regulation of normal human T cell blast activation: roles of APO2L/TRAIL, FasL, and c- FLIP, Bim, or Bcl-x isoform expression. J Leukoc Biol, 77(4), 568-78.

2. Del-Rey M, Ruiz-Contreras J, Bosque A, Calleja S, Gomez-Rial J, Roldan E, Morales P, Serrano A, Anel A, Paz-Artal E, Allende LM (2006). A homozygous Fas ligand gene mutation in a patient causes a new type of autoimmune lymphoproliferative syndrome. Blood, 108(4), 1306-12.

3. Bosque A, Marzo I, Naval J, Anel A (2007). Apoptosis by IL-2 deprivation in human CD8+ T cell blasts predominates over death receptor ligation, requires Bim expression and is associated with Mcl-1 loss. Mol Immunol, 44(6), 1446-53.

4. Bosque A, Aguilo JI, Alava MA, Paz-Artal E, Naval J, Allende LM, Anel A (2007). The induction of Bim expression in human T-cell blasts is dependent on nonapoptotic Fas/CD95 signaling. Blood, 109(4), 1627-35.

B. Modeling HIV-1 latency in primary cells. 

1. Bosque A, Planelles V (2009). Induction of HIV-1 latency and reactivation in primary memory CD4+ T cells. Blood, 113(1), 58-65. PMCID: PMC2614643.

2. Bosque A, Famiglietti M, Weyrich AS, Goulston C, Planelles V (2011). Homeostatic proliferation fails to efficiently reactivate HIV-1 latently infected central memory CD4+ T cells. PLoS Pathog, 7(10), e1002288. PMCID: PMC3188522.

3. Martins LJ, Bonczkowski P, Spivak AM, De Spiegelaere W, Novis CL, DePaula-Silva AB, Malatinkova E, Typsteen W, Bosque A*, Vandekerckhove L*, Planelles V* (2016). Modeling HIV-1 latency in primary T cells using a replication-competent virus. AIDS Research and Human Retroviruses. Feb;32(2):187-93. *Corresponding author.

4. White CH, Moesker B, Beliakova-Bethell N, Martins LJ, Spina CA, Margolis DM, Richman DD, Planelles V, Bosque A*, Woelk CH* (2016). Transcriptomic Analysis Implicates the p53 Signaling Pathway in the Establishment of HIV-1 Latency in Central Memory CD4 T Cells in an In Vitro Model. PLoS Pathog. 12(11): e1006026. *Corresponding author.

C. Discovery and characterization of HIV Latency Reversing Agents (LRAs). 

1. Novis CL, Archin NM, Buzon MJ, Verdin E, Round JL, Lichterfeld M, Margolis DM, Planelles V, Bosque A (2013). Reactivation of latent HIV-1 in central memory CD4+ T cells through TLR-1/2 stimulation. Retrovirology, 10(1), 119. PMCID: PMC3826617.

2. Pache L, Dutra MS, Spivak AM, Marlett JM, Murry JP, Hwang Y, Maestre AM, Manganaro L, Vamos M, Teriete P, Martins LJ, Konig R, Simon V, Bosque A, Fernandez-Sesma A, Cosford ND, Bushman FD, Young JA, Planelles V, Chanda SK (2015). BIRC2/cIAP1 Is a Negative Regulator of HIV-1 Transcription and Can Be Targeted by Smac Mimetics to Promote Reversal of Viral Latency. Cell Host Microbe, 18(3), 345-53. PMCID: PMC4617541.

3. Spivak AM, Bosque A, Balch AH, Smyth D, Martins L, Planelles V (2015). Ex Vivo Bioactivity and HIV-1 Latency Reversal by Ingenol Dibenzoate and Panobinostat in Resting CD4+ T Cells from Aviremic Patients. Antimicrob Agents Chemother, 59(10), 5984-91. PMCID: PMC4576101.

4. Bosque A*, Nilson KA, Macedo AB, Spivak AM, Archin NM, Van Wasgoner RM, Martins LJ, Novis CL, Szaniawski MA, Ireland CM, Margolis DM, Price DH, Planelles V* (2017). Benzotriazoles reactivate latent HIV-1 through inactivation of STAT5 SUMOylation. Cell Reports. 18 (5),1324–1334. *Corresponding Author

D. Role of HIV accessory genes to pathogenesis. 

1. DeHart JL*, Bosque A*, Harris RS, Planelles V (2008). Human immunodeficiency virus type 1 Vif induces cell cycle delay via recruitment of the same E3 ubiquitin ligase complex that targets APOBEC3 proteins for degradation. J Virol, 82(18), 9265-72. * Co-first author. PMCID: PMC2546900.

2. Ramirez PW*, Famiglietti M*, Sowrirajan B, DePaula-Silva AB, Rodesch C, Barker E, Bosque A, Planelles V (2014). Downmodulation of CCR7 by HIV-1 Vpu results in impaired migration and chemotactic signaling within CD4(+) T cells. Cell Rep, 7(6), 2019-30. PMCID: PMC4080720.

3. Ramirez PW, DePaula-Silva AB, Szaniawski M, Barker E, Bosque A, Planelles V (2015). HIV-1 Vpu utilizes both cullin-RING ligase (CRL) dependent and independent mechanisms to downmodulate host proteins. Retrovirology, 12, 65. PMCID: PMC4517359.

4. DePaula-Silva AB, Cassiday PA, Chumley J, Bosque A, Monteiro-Filho CM, Mahon CS, Cone KR, Krogan N, Elde NC, Planelles V (2015). Determinants for degradation of SAMHD1, Mus81 and induction of G2 arrest in HIV-1 Vpr and SIVagm Vpr. Virology, 477, 10-7. PMCID: PMC4455942.